Part:BBa_K2533014

RBS-TH-RBS-gldA-RBS-LLDP

produce and release lactate.

Characterization

This is a three section for lactate produce and transfer part.

Figure1. TH-gldA-lldP

Metabolic figure

Figure2. Metabolic figure of cyanobacteria with TH-gldA-lldP

With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with TH-gldA-lldP absorb CO₂ into Calvin cycle and produce NADPH. And then TH can transform NADPH into NADH which will be transformed into NAD+ through gldA. On the other hand, gldA can also turn pyruvate into lactate which will be transferred to the environment with the help of lldP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 68
  Illegal NgoMIV site found at 569
  Illegal NgoMIV site found at 944
  Illegal NgoMIV site found at 1007
  Illegal NgoMIV site found at 1604
  Illegal NgoMIV site found at 2612
  Illegal NgoMIV site found at 2978
  Illegal AgeI site found at 1616
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2240